In vitro analysis of antibacterial activity against wound pathogens, potential for wound healing, and anti‐melanoma properties of biosynthesized zinc oxide nanoparticles

Abstract Staphylococcus aureus is the most common cause of wound infections. Infected wounds increase severity and have a slower rate healing. Moreover, emergence multiple‐drug resistant bacteria such as methicillin‐resistant S. (MRSA) limited treatment options. This study was therefore aimed to evaluate antibacterial activity against pathogen wound‐healing properties green synthesized ZnO nanoparticles derived from mangosteen peel crude extract (ZnO‐Gm). their anti‐skin cancer also investigated in vitro. As result, ZnO‐Gm particles significantly inhibited growth MRSA with IC 50 values at 0.44 0.51 mg/mL, respectively. By performing quantitative reactive oxygen species (ROS) assay, intracellular ROS both treated found be elevated. Furthermore, exhibited cytotoxic effects via induction apoptosis on A375 melanoma cell line, an value 8.91 µg/mL, while not affecting normal line (Vero). In addition, 30 µg/mL could strongly promote healing epidermal keratinocyte (HaCaT). Consequently, findings this demonstrated that potential agents, materials, anti‐melanoma agents.